What is the purpose of buffer PB?
Buffer PB is used in DNA cleanup procedures and enables efficient binding of single- or double-stranded PCR products to the spin-column membrane. Buffer QG is a solubilization and binding buffer (with pH indicator), for use in DNA cleanup procedures.
What is in Qiagen elution buffer?
Elution Buffer QLE of the QuickLyse Miniprep Kit contains 10 mM Tris-Cl and 0.1 mM EDTA (pH 8.5). Due to the very low concentration of EDTA, enzymatic downstream reactions such as PCR and cycle sequencing are not inhibited.
What is the purpose of buffer PE?
Buffer PE is for removal of excess salt from the membrane. After addition of Buffer PE, you again apply vacuum. After removing salts, you transfer the column to a provided collection tube and centrifuge. Buffer PE contains ethanol.
What is QG buffer?
QIAquick Kits contain a silica membrane assembly in a spin column for binding of DNA in high-salt buffer and elution with low-salt buffer or water. QG buffer contains a pH indicator which allows monitoring any change in pH that would ultimately affect the binding of DNA to the silica membrane.
What is in Qiagen P1 buffer?
The composition of Buffer P1 is: 50 mM Tris·Cl, pH 8.0. 10 mM EDTA. 100 µg/ml RNase A.
What is Qiagen EB buffer?
Buffer EB is the elution buffer used in the QIAquick PCR, Gel Extraction, Nucleotide Removal Kits, and MinElute Kits for DNA cleanup, and the QIAprep Miniprep Kits for small-scale plasmid purification.
What is in Qiagen buffer EB?
The composition of Buffer EB is: 10 mM Tris-Cl, pH 8.5.
Is Qiagen elution buffer TE?
Buffer TE is a commonly used DNA resuspension and storage buffer. It is supplied in QIAGEN’s Endofree Plasmid Kits, and used for plasmid DNA resuspension in combination with other QIAGEN Plasmid Kits. Details on buffer preparation and storage are presented in Appendix B of the QIAGEN Plasmid Purification Handbook.
Why is ethanol added to the PE wash buffer?
The main role of monovalent cations and ethanol is to eliminate the solvation shell that surrounds the DNA, thus allowing the DNA to precipitate in pellet form. Additionally, ethanol helps to promote DNA aggregation. Usually, about 70 percent of ethanol solution is used during the DNA washing steps.
What is AW1 and AW2 buffer?
AW1 and 2 are wash buffers supplied as concentrates, AW1 contains is a stringent wash with low concentration of quanidine and AW2 is a Tris-based etanol solution to remove salts.
What is QIAGEN EB buffer?
How do you make a QIAGEN buffer P1?
Buffer P1 – Resuspension Buffer Prep – Dissolve 6.06g Tris base, 3.72g EDTA-2H20 in 800mL dH20. Adjust the pH to 8.0 with HCl. Adjust the volume to 1 liter with dH2O. Add 100mg RNase A per liter of P1.
What is included in the qiaquick PCR purification procedure?
The QIAquick PCR Purification procedure removes primers, nucleotides, enzymes, mineral oil, salts, and other impurities from DNA samples (see figure “Complete primer removal after PCR”).
What is the qiaquick protocol used for?
The QIAquick and MinElute systems use a simple bind-wash-elute procedure with spin columns or a vacuum manifold. The protocol has been used successfully for Cy3-, Cy5-, and biotin-labeling of cDNA from <50 ng of total RNA or poly A + mRNA.
What is the purpose of purification in PCR?
The purification procedure removes primers, nucleotides, enzymes, mineral oil, salts, agarose, ethidium bromide, and other impurities from DNA samples (see figure ” Complete primer removal after PCR “). Silica-membrane technology eliminates the problems and inconvenience associated with loose resins and slurries.