What is M-MLV?
M-MLV (Moloney Murine Leukemia Virus) reverse transcriptase is a DNA polymerase that uses single-stranded RNA, DNA, or an RNA-DNA hybrid (using a primer) to synthesize a complementary DNA strand. M-MLV is used for the preparation of cDNA libraries or for first strand cDNA synthesis for use in RT-PCR reactions.
How does Oligo dT work?
Oligo(dT)12-18 Primer is suitable for use in first-strand cDNA synthesis with reverse transcriptase. The primer hybridizes to the poly(A) tail of mRNA. It is phosphorylated on the 5´ end to facilitate cloning of cDNA. Performance and Quality Testing: Performance is evaluated in a first-strand cDNA synthesis reaction.
What is m-MLV reverse transcriptase?
M-MLV Reverse Transcriptase is a recombinant DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid. Compared to AMV RT, Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) lacks DNA endonuclease activity and has a lower RNase H activity.
What is first strand synthesis?
The SuperScript® First-Strand Synthesis System for RT-PCR is optimized to synthesize first-strand cDNA from purified poly(A)+ or total RNA. The system can be used with as little as 1 ng or as much as 5 µg of total RNA.
What is anchored Oligo dT?
Anchored Oligo(dT)20 Primer is a primer mixture consisting of a string of 20 deoxythymidylic acid residues followed by dV (either dG, dA, or dC) and then by dN (dA, dT, dG, or dC). Anchored Oligo(dT)20 Primer is recommended for use in cDNA labeling protocols and in RT-PCR.
What is the function of Oligo dT in reverse transcriptase PCR reaction?
Oligo(dT) primers amplify only mRNAs containing a poly(A) tail, since that is where the primer binds to promote reverse transcription. Random primers amplify most RNA species, including degraded RNA and viral genomes.
How much is reverse transcriptase?
Maxima Reverse Transcriptase (200 U/µL)
Catalog Number | Unit Size | Price (USD) |
---|---|---|
EP0741 | 2,000 units | 67.50 Your Price: Sign In |
EP0742 | 10,000 units | 209.00 Your Price: Sign In |
EP0743 | 4 x 10,000 units | 680.00 Your Price: Sign In |